FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_8404.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_8404.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23693866
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC484280.20439045278638784No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG339530.14329869173734672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCT96400.057.3859633
CGATCTA125550.048.2778324
ATCTATT147800.045.525016
CTCGATC144150.038.528612
CTATTAG198650.036.3082778
CGAAATG129550.031.125517145
TGTCCTA160150.029.7450055
GATCTAT239200.027.31115
AACGTAT611350.026.7799996
TATTAGT277700.026.2599099
GATCCTG178900.025.3711175
ACGAATC67050.025.0875572
GTATTCA662650.024.9852099
CGTATTC663600.024.88398
GGAACGT616350.024.5853864
CCGGGAA598300.023.9992121
AGTCACA72350.023.656618145
TGCCGTA176600.023.6430159
CGGGAAC621350.023.4779872
GAACGTA719850.022.9953215
Produced by FastQC (version 0.11.5)