FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_8336.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_8336.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23340594
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTATG256850.032.556595145
CCGGGAA232750.025.2055361
CGGGAAC296050.023.3924331
GAACGTA337650.022.0528374
AACGTAT327300.021.7100735
GGGAACG316550.021.5761532
GGAACGT324900.020.8875223
ACGTATT357250.020.1133126
TATTCAC345450.020.0816369
TGTCCTA100850.019.7714275
GTATTCA378100.018.2708448
CGTATTC402050.017.4709347
CCTACTC154800.017.3261938
CTACTCT135050.016.0490369
CGATCTA62300.015.0128254
CTGAACA253450.014.307605145
TCCTACT159650.013.6214937
AGCTATA277850.013.364378145
CGTGCTA133550.012.2157435
GCGGTGT232350.012.1719181
Produced by FastQC (version 0.11.5)