FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_8336.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_8336.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24334133
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGC41250.038.6603627
GACATAC55650.038.4259769
TATGATG148450.033.270336145
TGCCAGT136650.030.5635662
GTAGCAT103800.029.3304737
GTTACGA100500.029.0749844
AACGTAT328750.027.0849656
GAACGTA330700.027.0786845
AACAGAC81450.026.172855
ACGTATT354650.025.2834847
GCCAGTA171650.024.711233
TACGACT120050.024.2806076
CAATTAG67750.022.361719
CCGGGAA366150.022.341841
TAGGCTC76650.022.2240779
CGTATTC421500.021.9614248
CGGGAAC391700.021.5471882
GCAAACT111450.021.4041543
CTTGTTA140300.020.8312931
TGTACTC63450.020.6841722
Produced by FastQC (version 0.11.5)