FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_6331.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_6331.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22797153
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG119500.041.9883775
CTCATAT50600.033.5364571
CAATTAG121600.032.4885259
ACGAGCA101800.031.1247224
TCACGAG106900.030.0474342
TCGAGCT66550.028.5448593
TTTGATC193350.027.0753692
CTGGCTC199250.026.084829
CATATCA63700.025.1551883
CTATTAG143950.024.9768148
GAGCAAT146250.024.838926
GGCTCAG166750.024.7410564
TCCTGGC199700.024.6466987
CCTGGCT197600.024.5784728
TCATATC72950.023.9537282
ATTGAAC121450.021.8511566
TGATCCT223350.021.750074
ATCCTGG248550.021.7336866
TCAGATT114650.021.375882
TATTAGT187850.020.837699
Produced by FastQC (version 0.11.5)