FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_6215.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_6215.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22187364
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2804341.2639356347153272No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT745610.33605163731933185No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT695950.31366952829547484No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT328810.1481969647228035TruSeq Adapter, Index 19 (97% over 38bp)
GTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256360.11554324344252881No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTTT410950.029.5936181
GCCTAAC113500.029.4550021
CCTAACT125000.027.8998532
GCCATAT33050.026.1113621
GAGTCTA47800.026.0891284
TCTCTTG77450.024.5278515
TCAGATT124800.023.529212
GTACTCT114350.022.00163
GATCCAA97100.021.6550085
CGTTTTT79350.020.745931
TAGTTGT125350.020.2979749
TGCTTGG155500.020.2781728
CTAACTG156500.019.8285263
GCTTGGA161650.019.2824739
GGATCTC100750.018.9268172
TCATTAG71250.018.825563
TAACTGC175200.017.9189624
ACACGTA35200.017.719437145
TCTAGTT162050.017.5797827
CGGGTTT222050.017.5378971
Produced by FastQC (version 0.11.5)