FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_5764.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_5764.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22807312
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC272170.11933453622241849No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT243000.10654477827110884No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC228490.10018278348627845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT466850.029.6810116
CCGGGAA423250.027.9456541
ACGTATT511850.027.3188887
CCATTGT336800.027.02366145
GAACGTA523350.026.739945
CGTATTC551400.025.5171578
GTATTCA564550.024.7943869
GGAACGT512200.024.3625834
CATTGTA223450.023.855003145
TATGATG208250.023.43703145
GGGAACG550950.022.8859773
CGGGAAC562350.021.403622
GGGTTAG214000.019.4821173
CGATTAG97650.018.6326668
GGTTAGA245650.018.4183144
GATTAGT103300.018.0345929
ATCGATT102400.017.6317256
TGGGCTA181700.017.3588182
TATTCAC534800.017.2954869
GTTAGAG106300.016.8484155
Produced by FastQC (version 0.11.5)