FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_31013.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_31013.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15943437
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2746331.7225457722823505No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524150.3287559639743927No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479000.3004371014857085No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT345900.21695447474719534TruSeq Adapter, Index 19 (97% over 38bp)
GTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176840.11091711279067368No Hit
CGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171090.10731061313818344No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC169830.10652031930128993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAC69400.041.6953351
CCTAACT80100.036.8439672
TCTAGTT89850.032.4326977
CGATTAG46350.031.9047668
ATCGATT49800.031.8884146
GTACTCT89850.031.8765473
CTTTTTT293350.031.6444761
TACTCTA95150.030.9391334
TAGTTGT93850.030.0463529
TAACTGC99000.029.0767734
TTGTACT99000.028.6428071
TCGATTA56300.027.6824917
TGTACTC104700.027.3561482
GTTACTA41450.026.7645023
GAGTCTA25250.026.4198862
GCTTGGA113600.026.0988549
ATCCAAC79600.025.5072176
TCAGATT52050.024.7972662
TCATTAG49800.024.3152853
GATCCAA74100.023.6817595
Produced by FastQC (version 0.11.5)