FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30982.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30982.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21469544
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG676000.3148646287038048No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC597070.2781009228700898No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA420330.19577965885069568No Hit
CCTCGATCTATTAGTACCAGTCAGCTCCAAACCTCACGGCTCTTCCACAC326940.15228083092961825No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA317110.1477022520832301No Hit
CTGATATCGACCGATAGCGCATAGTACCGTGAGGGAAAGGTGAAAAGAAC273540.12740838836632953No Hit
AATCAAACGAGATTTTCTTAGTAGCGGCGAGCGAACGGGAAAGAGGCCAA254190.11839562125772209No Hit
GGAAAAGTAATCAAACGAGATTTTCTTAGTAGCGGCGAGCGAACGGGAAA224880.10474372441259115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCT69050.079.592983
CTCGATC74200.073.679012
GCAATTA113450.062.3623548
CGATCTA98600.058.7542274
ATCTATT112500.052.6577226
ACGAGCA126150.052.360094
GATCCTG105250.050.70475
TATTAGT146800.049.4293869
GAGCAAT147700.047.9139336
AGCAATT150150.047.216167
CTATTAG144800.047.0583238
CGAGCAA154000.046.00095
CAATTAG155950.045.7390259
CGAGAAC154550.043.4512521
TCACGAG181050.039.326862
GGGCACA20500.038.205881
GGCTCAG143800.037.563554
TCAGATT68950.037.217417
CGCTGAT61850.036.348151
GCTCAGA74950.035.0210725
Produced by FastQC (version 0.11.5)