FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30979.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30979.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22127725
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG505750.22855942036517535No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA449270.2030348804497525No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC346060.15639203759085038No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG341280.15423185167024628No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT328230.14833427295395257No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC249490.11274995509027702No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG248140.1121398607403156No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC226790.10249133157611097No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT222090.10036729939476381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGA182600.040.7404374
TTCGTTC161150.035.9855239
GGGTTAG208700.035.7150083
AACGTAT367350.035.5493285
TGGGTTA211000.033.2986222
TATTCAC398900.032.8187379
GTATTCA399200.032.6306538
GAACGTA415200.032.184524
ACGTATT409350.032.0967526
GGAACGT421200.030.3661883
CGGGAAC415450.030.3390081
CTCCGAC190100.027.1242561
TCACACG96950.026.7672278
GTTAGAA269400.026.7538645
TTGAACG94900.025.9705337
GAACCTC272400.025.759449
TTTCGTT226700.025.4844328
CTCGATC153550.025.3154341
GAAACTA214550.024.9406034
TATCCGG216900.024.7977649
Produced by FastQC (version 0.11.5)