FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30978.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30978.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21729389
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT220260.10136502227467141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCA75800.033.192614
CGAGCAA97200.027.4525495
TCACGAG100450.024.2535022
CAATTAG142200.020.7983469
CGAAATG144150.019.874426145
ACACGTA47000.018.826696145
GTTACGA59100.018.6482144
GAGCACC102450.018.335804145
TACGACT62800.018.2431416
GAGCAAT156700.018.1392466
AACGTAT193800.017.5477436
GATCCCG193500.017.2368553
TACGGAC187000.017.0613045
TTACGAC70450.016.1591975
ATCCCGA208300.016.046964
ACGTATT210900.015.9824957
GAACGTA216550.015.7711685
CGCGAGC93450.015.67581751
CTACGGA216150.015.3970884
CGGGAAC230600.014.7153872
Produced by FastQC (version 0.11.5)