FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30978.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30978.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24229767
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC57800.040.1315969
GAGTCTA78500.036.6689952
GTTACGA90900.035.2558444
TTACGAC102350.031.3832245
TGCCAGT119500.031.3083292
AGTCTAA95250.029.915843
GTAGCAT126050.029.8461847
AACAGAC81500.029.1808515
TAGCATA130250.028.9950778
AGTCACA75550.028.79998145
TGTTACG119050.026.7976633
TACGACT123400.026.6174016
TGTACTC90250.026.4318032
TTGTACT89250.026.0827241
GCCAGTA147950.025.826723
ACACGTA49900.024.563578145
GTCTAAC118900.024.1482734
TCTAACA121750.023.7621445
CCAGTAG161350.023.7267194
GAACGTA135100.023.4535065
Produced by FastQC (version 0.11.5)