FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30976.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30976.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21985385
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493430.22443546019321475No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT363580.165373496984474No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT326580.14854413511521405No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT322900.14687029588064981No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC269500.12258143307474487No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA253890.11548126175638952No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC220710.10038941778822615No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA220350.10022567264571441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATG130450.043.58627145
GACATAC45900.039.7979859
GAGTCTA67900.038.5490262
GTAGCAT89100.034.088637
TAGCATA89250.033.7876788
AGTCTAA78100.033.328023
TGCCAGT90750.032.5179942
TTACGAC75300.032.4500855
GTTACGA74550.032.3862534
GGCCAAC192150.030.6836591
CCAGTAG99550.030.3709874
ACACGTA35950.028.444477145
AACCGAG226000.028.3932915
AACAGAC66700.027.7201275
TCTAACA93650.027.7176345
GTCTAAC94600.027.5148664
GCCAGTA110000.027.4858683
CCAACCG226000.026.4353373
TACGACT94500.026.2406866
CGCTCAG66800.025.723854