FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30908.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30908.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23883387
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA481390.20155851429280108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATG336650.014.800439145
TATGCCG304450.012.792481145
CGTATGC365850.012.033228145
CTCGTAT380300.012.014638145
ATCTCGT474150.012.00744145
ACACGTA82200.011.558354145
TCGTATG405950.011.309091145
GTATGCC361650.011.150204145
GATCTCG525300.010.5483145
CGGCTAT1022850.010.352313145
TCTCGTA423400.010.123557145
ATGCCGT331150.010.096542145
TCACCGG1365400.09.975443145
CCGGCTA1150650.09.807602145
GGCTATG1013800.09.800873145
ACCGGCT1189850.09.783164145
CCCGAAT95900.09.7551291
TGATCTC657450.09.586356145
GGGGAAT198300.09.5816551
GTCACCG1476500.09.504823145
Produced by FastQC (version 0.11.5)