FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30904.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30904.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21562213
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1308960.6070619931265867No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG306400.14210044210211634No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT278410.12911939975734402No Hit
GGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGG278240.12904055812824036No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC268560.12455122301222049No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC255450.1184711420854622No Hit
CTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTTGATTTTCCGGA229090.10624605183150729No Hit
CGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGA221780.10285586178005013No Hit
CTTAACCATGACTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCT221650.10279557112250028No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218530.10134859534130379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT64550.076.839236
TCGATTA66450.074.7239157
CGATTAG69150.071.911118
GATCGAT69350.071.3117455
TCGATCG79650.061.08633
CGATCGA82750.059.6741374
TCAGATT58850.052.49022
TTGAACG59300.052.1960037
TATGATG72450.052.1538145
GATTGAA59300.051.3591465
CCTAACT84550.050.2572942
GATCCAG126750.049.4298135
GCGACTA31100.048.4973562
ATCCAGC130850.047.9918336
GATCCAA67200.047.2637335
ACACGTA17750.046.98791145
CAGATTG66850.045.448513
GGAAGCA107450.044.6069033
ATCCAAC75150.043.325196
TCATTAG94250.041.160393