FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30903.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30903.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22843571
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATG190050.041.90109145
GACATAC84500.039.204469
AACAGAC99850.033.5488245
TGCCAGT111200.025.2335952
GTAGCAT112250.025.185717
TAGCATA112250.024.9273958
CGGATGT69100.023.823491
AGACATA166150.023.2979058
GTTACGA89850.023.2400914
CTCCAAC171750.022.2521231
TCCAACA155800.021.9192852
GAGTCTA46900.021.7976534
TTGTACT85450.021.5565471
TGTACTC90850.020.4309232
TTACGAC104450.020.0619355
CAACAGA173000.019.8653244
CTGCCAG141700.019.8572651
CCAGTAG141350.019.6456494
TCACAGC93100.018.4574452
CTACTCT121550.018.3087799
Produced by FastQC (version 0.11.5)