FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30893.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30893.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20697435
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT402730.19457966651423234TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG177250.050.206142145
CGTCTTG181500.047.871685145
TCTCGTT184650.042.53806145
GCGCATG323400.040.793518145
ATGCCGG206850.032.53801145
CCGGGAA162700.028.6162971
CGGGAAC208050.024.8186531
AACGTAT215850.023.246755
GGGAACG223850.022.5777022
AGTCACA176200.022.515413145
GAACGTA234750.022.486264
GGAACGT219700.022.3764973
CTTCTGG284850.021.616728145
CGTCTTA85150.021.464184145
CGCATCG322550.021.3162145
CTTACAT108250.020.7012481
GCATCTG178100.020.361258145
ACGTATT251850.019.7510596
CGTATGG103000.018.37815145
TATTCAC270550.018.272739
Produced by FastQC (version 0.11.5)