FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30893.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30893.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19512136
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC484940.24853250305348426No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC279740.14336718440256874No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT270170.13846254454150997No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG257070.13174877419878583No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT252770.12954501752140307No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGC209530.10738445037488463No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG204860.10499106812293642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA491000.035.2553521
CCATTGT387550.033.421482145
GATCCTG76450.033.196425
AACGTAT606000.032.0673376
CAATTAG80150.031.4741489
GCTGTAT44050.031.1150171
GAACGTA658350.029.660625
ACGTATT672600.029.1102547
CTATTAG64850.028.9512988
CGTATTC702600.028.011738
GGGAACG672150.027.6263853
TCAGATT124150.027.2748952
CGGGAAC659100.026.9420362
GGAACGT707900.026.354124
GTATTCA755700.026.321649
CATTGTA254250.026.299162145
GTTAGAG145700.025.7792875
GGCTCAG120000.025.7400284
TACTACT37150.024.9834186
CGAAATG173850.024.94586145
Produced by FastQC (version 0.11.5)