FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30890.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30890.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23535881
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG454300.19302442938082498No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC380730.1617657737137607No Hit
CTCAGGAACCCTTAGGCTTTCGGTGGAATGGATTCTCACCATTCTTTTCG237960.10110520188303126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA50050.048.5302664
TGCCGTG149200.045.159195145
TGTCCTA123350.039.7957885
GCGCATG269350.039.66296145
CTATTAG106750.039.1130188
CGTCTTG145650.036.151424145
CCGGGAA294550.035.2077671
CGGGAAC402150.033.4155651
GGAACGT393900.033.3537833
GGGAACG415450.032.6188742
GTGTCCT154150.032.3606224
CCTACTC157550.032.344748
AACGTAT473150.032.1050075
AGTCACA124950.030.41546145
CTACTCT175050.029.0283729
GTATTCA527000.028.6926388
TATTCAC535000.028.358439
GAACGTA563550.028.1505454
TGGGCTA159850.026.5805152
CGTATTC572900.026.4444317
Produced by FastQC (version 0.11.5)