FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30890.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30890.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20355190
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA406900.19989987811462334No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT375240.18434610534217563No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT346400.1701777286284235No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT281900.13849047834974765No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA250530.12307917538475445No Hit
CCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCG231360.11366142983681311No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC220140.10814932211391788No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA220060.10811002009806835No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA214970.10560942933964261No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT213200.10473987223897198No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA212190.10424368428887179No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG207610.10199364388148674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATG132850.041.981895145
CGGATGT33800.041.839281
GACATAC45950.037.7030039
CTCGGCT80550.034.662631
TGCCAGT82000.033.4245262
GAGTCTA51650.032.9901922
CGCTCAG74450.032.8205684
GAGCACC81350.031.025509145
GGCCAAC147100.030.517171
AACAGAC56850.029.8460315
TTACGAC66350.029.1790735
ACACGTA40000.028.648003145
GCCAGTA95500.028.6232453
GTCTAGG22800.028.6225856
TCTAGGC22550.028.2878327
TAGCATA87700.027.8544038
GTTAGAG85700.027.4135555
CATGAAT112500.027.3325161
GTAGCAT90550.026.9777057
CCAGTAG103800.026.5440484
Produced by FastQC (version 0.11.5)