FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30889.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30889.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22829979
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC53000.035.6977849
TATGATG152200.033.594116145
GAGTCTA72750.033.388132
TTACGAC78650.031.4374545
GTTACGA81200.030.4489944
TTGTACT116450.030.2659381
TGTACTC122550.029.7009752
AGTCTAA88700.027.3022463
TAGCATA112000.026.5364278
GTAGCAT115100.025.6957597
CCAGTAG117300.025.590394
TCTAGTT155100.025.1914757
TGTTACG98250.025.1650243
GTCTAAC100950.023.9891434
TGCCAGT120000.023.927332
AACAGAC81400.023.7836535
TCTAACA106250.022.7934075
TACGACT112450.022.7618686
ACACGTA45100.022.513357145
GAACGTA133350.021.7499715
Produced by FastQC (version 0.11.5)