FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_30887.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_30887.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20505104
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1956980.9543867712155959No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440960.21504889709410888No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372830.18182302318486168No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG249970.12190623368698837No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG226500.11046030295676627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT64100.048.5274476
CGATTAG71600.042.4205068
GATCGAT78750.040.0522465
CCTAACT81100.038.8015062
TCGATTA78950.038.746747
GCCTAAC81900.037.9860461
GTACTCT71150.036.5841453
TCATTAG66700.036.0898933
TCTAGTT72700.034.9983527
TAGTTGT72400.034.4425059
ACACGTA20300.033.225254145
TACTCTA80100.033.129964
CCACACA105550.031.6681272
TTGTACT77650.031.6598551
TCGATCG101750.031.2113863
TGTACTC80700.031.1770952
GGAAGCA103200.031.1241423
TAACTGC100400.030.9810314
TTGAACG55750.030.5560957
GTTACTA35300.029.7828773
Produced by FastQC (version 0.11.5)