FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_2397.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_2397.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21522125
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2724041.2656928625774637No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT774910.3600527364282105No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT704310.32724928416687477No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG298330.13861549452017402No Hit
GTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248170.1153092457180692No Hit
CGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234960.10917137596775411No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC228690.10625809486749102No Hit
CTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTTGATTTTCCGGA220070.10225291415229677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG57650.048.4102978
ACTCTAG109050.044.0194745
CCTAACT96500.041.853462
GCCTAAC97550.041.4859731
CTCTAGT116150.041.2662286
TCGATTA70500.040.1006857
TAGTTGT120300.039.3481149
TCTAGTT124000.038.8755237
ATCGATT75050.038.550926
GTACTCT126000.037.751113
TACTCTA133050.036.0230944
TTGTACT138400.034.9005621
TGTACTC139400.034.2268262
GGAAGCA145050.033.7429053
TCATTAG85100.033.6561133
GATCGAT88650.033.3729025
ACACGTA25200.033.096073145
TAACTGC122450.032.80544
CTTTTTT409450.031.4938091
TCGATCG93800.030.3025823