FastQCFastQC Report
Mon 20 Nov 2017
HMHTMBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHTMBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356925290
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23153910.6487046630963023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGG840950.018.1995166
TATACCG923300.017.769265
GTTATAC1027500.016.8232423
TTATACC994650.016.5268864
GTCATAT1710400.015.0035511
TACCGGT1005600.014.837277
GTAGATC1588900.014.68779543
GTATCAA2031300.014.4343771
GGACCAT848050.013.5807246
TGTTATA1510350.013.3583242
CCTCGTT1354600.013.20477112
TAGCCTC1495550.013.03642759
TATAGCC1541650.012.9070857
AACGCAG1887550.012.76462656
TACACCG651050.012.6809485
TCAACGC1965450.012.65947153
CGTTTAA1342600.012.65043915
TAGTCCA593900.012.6447199
ACCGGTG1234200.012.5521028
GGTATTC940000.012.53724124
Produced by FastQC (version 0.11.2)