FastQCFastQC Report
Mon 20 Nov 2017
HMHTMBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHTMBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356925290
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCC9799100.013.9051125
CCTCTCT4666300.013.8934144
TCGCATC9823900.013.8833354
TCAACCG3793100.013.8465731
GTCGCAT9853300.013.8462673
CCTTCTT3864250.013.8416971
CTCCTAC3648000.013.8393984
TACTCCA4336350.013.8372523
ACACCAT5194650.013.8371343
TAAGGAT8461850.013.8331022
CTTCAGC5222550.013.8281161
CCACACT6173050.013.8272042
TTGTCGC9801150.013.8268561
CTACTCC4346000.013.8197182
TACACCT3838900.013.8181831
GCATCCA9854400.013.8174396
CCTACAA3052050.013.8170611
TCAGAAC3770250.013.8141282
GTAAGGA8469000.013.8075211
ACCTCTC4667050.013.8036133
Produced by FastQC (version 0.11.2)