FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28632967
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16806845.869751465155533No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT417880.1459436599776754No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN400670.13993310577978174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTC173750.042.557739
GCATAGC203900.042.3696251
ACGAAAC232000.041.87949813
AGGACGA267050.041.80388310
ATAGCTC207500.041.78443
TCCGATT179550.041.7474148
CATAGCT206250.041.664662
CGAAACA225900.041.6425114
TAGCTCT206300.041.4901774
CGATTCC177600.041.35220310
AGTTGTC190050.041.269493
AACACCG201550.041.2093517
CGTTTCG64400.041.0474857
TAGGTCC102150.040.69972220
GATTCCC183450.040.48890711
GCATTCT189050.040.4030381
TCACCTA178000.040.1935779
TGTACCC147900.040.1000610
TTGTGGA329400.039.9862332
GTCCGAC40600.039.823974
Produced by FastQC (version 0.11.5)