FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_cDNA5_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_cDNA5_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44520511
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6216141.396241835588994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2319390.5209711092489482No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1051090.23609118053474273No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT476420.10701135034141905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT853600.037.1469041
AGCAGTG929500.034.1150632
ATCAACG993600.031.5918611
TCAACGC1003450.031.24437312
CAACGCA1010500.031.04373713
TGGTATC1025950.030.713117
GTATCAA1024400.030.6613379
AACGCAG1027800.030.59661114
GGTATCA1034550.030.4413348
ACGCAGA1031950.030.36435315
AGAGTAC1033700.030.06777219
CAGTGGT1059500.029.879244
TATCAAC1053200.029.86686710
CGCAGAG1056200.029.80894716
AGTGGTA1069100.029.5829945
GTGGTAT1071550.029.4714476
GAGTACT1046750.029.37947320
GCAGAGT1083450.028.8847417
GTACTTT1062350.028.82790222
CAGAGTA1087450.028.76821518
Produced by FastQC (version 0.11.5)