FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_cDNA5_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_cDNA5_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50061711
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7097831.417816103009344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2764080.5521345445024841No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1216530.24300607703959617No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT545940.10905340410758232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT968900.037.1938821
AGCAGTG1050550.034.2754252
ATCAACG1126150.031.64834611
TCAACGC1135050.031.3750212
CAACGCA1143150.031.19489113
GTATCAA1157750.030.8341189
TGGTATC1162700.030.7608017
AACGCAG1163100.030.6942414
GGTATCA1170850.030.5718148
ACGCAGA1168000.030.48598915
AGAGTAC1165850.030.38268719
TATCAAC1192600.029.97929810
CAGTGGT1198550.029.9772284
CGCAGAG1195200.029.88783316
AGTGGTA1211700.029.6347525
GAGTACT1183650.029.6207420
GTGGTAT1217200.029.4477966
GCAGAGT1217250.029.18544417
GTACTTT1199750.029.14206522
CAGAGTA1226700.029.00157418
Produced by FastQC (version 0.11.5)