FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_cDNA5_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_cDNA5_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36259687
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5393701.4875197350710723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2068950.5705923495699232No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT908050.25042963001859336No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT412350.11372133466016957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT723600.037.2379651
AGCAGTG783000.034.366872
ATCAACG847900.031.52764311
TCAACGC855750.031.21524812
CAACGCA861000.031.04514113
TGGTATC868050.030.8380327
GTATCAA870250.030.699999
AACGCAG875700.030.5924314
GGTATCA878400.030.4977638
ACGCAGA878800.030.4265515
AGAGTAC877900.030.07872619
TATCAAC892000.030.03552810
CAGTGGT897150.029.9868744
CGCAGAG898600.029.82965716
GTGGTAT907850.029.5825446
AGTGGTA908150.029.5811985
GAGTACT890000.029.3382920
GCAGAGT917350.029.00162717
CAGAGTA918950.028.91238418
GTACTTT900900.028.85374622
Produced by FastQC (version 0.11.5)