FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_cDNA5_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_cDNA5_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62853322
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9211661.465580450942593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3241320.5156958927326069No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1567060.24932015526561985No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT700610.11146745751958823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1268150.037.284361
AGCAGTG1373000.034.4177672
ATCAACG1478850.031.59727311
TCAACGC1490750.031.3183812
CAACGCA1498600.031.192513
TGGTATC1524200.030.8292647
GTATCAA1519950.030.8032789
AACGCAG1528800.030.65852414
GGTATCA1536150.030.5972588
ACGCAGA1536300.030.42979415
AGAGTAC1537250.030.29440719
TATCAAC1563000.030.00302510
CAGTGGT1581550.029.8269234
CGCAGAG1575400.029.81276916
AGTGGTA1589150.029.693655
GAGTACT1557450.029.63417220
GTGGTAT1591850.029.5809086
GCAGAGT1607150.029.10470217
GTACTTT1580350.029.0727222
CAGAGTA1612050.029.0692118
Produced by FastQC (version 0.11.5)