FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_cDNA3_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_cDNA3_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44015339
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1218640.2768671167112901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1840450.039.9079741
AGCAGTG2013200.036.337012
CAGTGGT2483400.029.3820294
AGTGGTA2535700.028.8975585
GCAGTGG2545300.028.7058533
GTGGTAT2593400.028.1777676
TCAACGC2609450.027.81960712
ATCAACG2622700.027.78883211
CAACGCA2619650.027.75402613
TGGTATC2645800.027.5577477
TATCAAC2670950.027.36319510
GTATCAA2666250.027.2891149
GGTATCA2675700.027.2610768
CGCAGAG2682050.027.17593416
GAGTACA2654500.026.9577920
GTACATG2632600.026.91176622
AGTACAT2656450.026.78467221
TACATGG2629050.026.75166323
GCAGAGT2698650.026.75020217
AGAGTAC2690250.026.72395119
Produced by FastQC (version 0.11.5)