FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_cDNA3_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_cDNA3_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45890449
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1225180.2669793010741734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG461870.10064621507625694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1935450.040.0516241
AGCAGTG2124100.036.3081782
CAGTGGT2624000.029.2683414
AGTGGTA2676500.028.7560335
GCAGTGG2686800.028.6318913
GTGGTAT2740900.028.0320246
TCAACGC2768100.027.6420212
ATCAACG2781950.027.5891511
CAACGCA2781200.027.54097213
TGGTATC2792650.027.44667
TATCAAC2835150.027.18860810
GTATCAA2827150.027.1742529
GGTATCA2826050.027.1678778
CGCAGAG2836350.026.98828116
GAGTACA2821050.026.82103220
GTACATG2789700.026.76769622
AGTACAT2824150.026.66217221
TACATGG2781450.026.61875223
AGAGTAC2850450.026.60574719
GCAGAGT2859350.026.58193417
Produced by FastQC (version 0.11.5)