FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_cDNA3_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_cDNA3_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69476999
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1779000.2560559646509775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG717280.10323992261093487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2938400.040.0560871
AGCAGTG3221550.036.3383872
CAGTGGT3976900.029.3634934
AGTGGTA4067100.028.7809815
GCAGTGG4073300.028.7045693
GTGGTAT4164250.028.0536486
TCAACGC4202400.027.68948712
ATCAACG4223500.027.6383311
CAACGCA4224450.027.57624813
TGGTATC4245600.027.4874697
TATCAAC4300100.027.24743810
GTATCAA4295300.027.2117149
GGTATCA4303150.027.149888
CGCAGAG4309000.027.01420216
GAGTACA4273400.026.86552620
GTACATG4232450.026.81375522
AGTACAT4278350.026.7047121
GCAGAGT4338600.026.64675117
TACATGG4228950.026.63453523
AGAGTAC4331600.026.61895419
Produced by FastQC (version 0.11.5)