FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_cDNA3_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_cDNA3_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44391928
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1188350.26769506384133623No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG447330.10076831986211549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1872000.039.983531
AGCAGTG2043800.036.4708672
CAGTGGT2532450.029.3613594
GCAGTGG2583450.028.7802373
AGTGGTA2591900.028.7439825
GTGGTAT2647500.028.0650836
TCAACGC2672150.027.71514112
ATCAACG2685050.027.67722311
CAACGCA2686200.027.61024913
TGGTATC2698900.027.4941237
TATCAAC2731650.027.31141910
GTATCAA2727150.027.235479
GGTATCA2733400.027.1618948
CGCAGAG2739750.027.04022616
GAGTACA2717350.026.90029720
GTACATG2693550.026.81574822
AGTACAT2718400.026.76559321
TACATGG2686950.026.67636123
AGAGTAC2753000.026.65457319
GCAGAGT2762350.026.63893517
Produced by FastQC (version 0.11.5)