FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_HTO5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_HTO5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8563618
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG448220.5234002731088659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACA2664850.043.8702241
TCACATC2663850.043.7997553
ATCACAT2678250.043.7963562
CATCGGT2667050.043.72356
TCGGTCC2635250.043.7027328
CACATCG2664150.043.6998064
ATCGGTC2660900.043.698317
CGGTCCC2626300.043.6723869
ACATCGG2678100.043.6676255
GGTCCCA2625450.043.63030210
CGTATTC1097500.043.6208727
CTAACGG1154450.043.6127246
CATGTTA688750.043.58552
GTATTCC1093000.043.5483678
CCGTCCC666450.043.541119
TAACGGC1151150.043.5201267
TTACTAT666450.043.5192644
ACGGCCC1136100.043.51149
TCTAACG1161350.043.509945
TATCCCC647250.043.4934048