FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_HTO3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_HTO3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10267248
Sequences flagged as poor quality0
Sequence length50
%GC17

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4613974.493872165160518No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4067443.961567890441528No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3972063.868670553199845No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3488273.3974732080105596No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2554742.4882422242065254No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2470032.405737155662355No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2441432.377881590081393No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2356172.294840837583742No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2135222.0796419839084437No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130242.074791609202388No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1944111.8935064196364986No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1791091.7444694040701072No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1701161.6568802078219986No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1571191.5302932197605434No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1548741.5084275747503129No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1465841.4276853933985036No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1376561.340729278186326No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1224931.1930460820660025No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1002770.976668723693048No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA838660.8168303716828501No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA812660.791507130245612No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790570.7699921147322047No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA345530.3365361389926492No Hit
AAGTATCGTTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324920.3164626002995155No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324750.316297025259349No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT311870.303752281039671No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT308450.3004213008198497No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT298870.2910906603210519No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267670.2607027705963662No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT236040.22989607341714155No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT196460.1913463081830691No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT193130.18810298533745362No Hit
CTCCTCTGCAATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA191430.18644723493578805No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT186240.18139232635658553No Hit
GGTTGCCAGATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182100.1773600871431176No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT177060.17245127418759146No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT176210.17162339898675869No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC175270.17070786641172006No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC173470.16895471892760358No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT172200.16771777598047694No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT172080.16760089948153584No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT169990.16556530045831172No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT160860.15667294683054311No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC160480.15630283791722963No Hit
TGTCTTTCCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA159570.15541652446692628No Hit
TTCCGCCTCTCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA158590.1544620330589073No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT155890.15183231183273257No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT153570.14957269951987134No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG143230.13950184119444664No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT142990.13926808819656444No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG138040.13444693261524412No Hit
TGTCTTTCCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131240.12782393100858186No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG130690.12728824705510183No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT130320.12692787785003343No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT124680.12143468239980179No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT124550.12130806619261558No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC122360.11917507008694053No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT116460.11342864222233649No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT115800.11278582147816046No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC114520.11153913882278874No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC112690.10975677221393698No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT112150.10923082796870202No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT111310.10841269247611435No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT108150.1053349446706654No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG104010.10130270545719748No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT103210.10052352879759015No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC102810.1001339404677865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGC4550.044.0042538
GGTTCGC1050.044.0042538
CTATGGG302.5280078E-644.004258
CCTTAGC207.855667E-444.004258
AGATCTC10350.044.004258
TCACCGT502.7284841E-1144.004258
CTGTAGC408.3164196E-944.004258
GTTTCTA1650.044.004258
CACGGTC408.3164196E-944.004258
TCTCTGT254.442784E-544.004258
GTTTATC650.044.004258
CTATACC454.802132E-1044.004258
AGAAGTC3050.044.004258
TCCCTTT207.855667E-444.004258
CCTGTCA1000.044.004258
CATTCGC408.3164196E-944.004258
AGAACTC351.4464968E-744.004258
TCGGCGT408.3164196E-944.004258
CGTTAGC254.442784E-544.004258
AGAAATC600.044.004258
Produced by FastQC (version 0.11.5)