FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_GO5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_GO5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14955240
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCCATATAAGAAAA13608019.099158555797166No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCCATATAAGAAAC13507609.032018209002329No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCCATATAAGAAAT12572238.406571877148076No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCCATATAAGAAAG10374006.936699110144671No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCCCATATAAGAAA2460171.645022079217719No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1356020.9067189827779426No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAC846950.5663232418871245No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAA826820.5528630767543684No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAC801600.5359994222760718No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAA779400.5211551268986656No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAT735610.491874419935755No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAT734890.4913929833289202No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAG727160.4862242264249855No Hit
GCATAGCTCTTAAACCTGTAGGAGCGGCGTTAGTACCCCATATAAGAAAA725010.48478660322402045No Hit
GCATAGCTCTTAAACCTGTAGGAGCGGCGTTAGTACCCCATATAAGAAAC712930.4767091668204589No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAG686660.4591434172905283No Hit
GCATAGCTCTTAAACCTGTAGGAGCGGCGTTAGTACCCCATATAAGAAAT661270.4421660902800623No Hit
GCATAGCTCTTAAACCTGTAGGAGCGGCGTTAGTACCCCATATAAGAAAG641800.4291472420369048No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAA615680.4116817918000647No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAC582480.38948221492934915No Hit
GCATAGCTCTTAAACCGTCATCGTTGAGGTGTTTACCCCATATAAGAAAA577200.3859516798125607No Hit
GCATAGCTCTTAAACCGTCATCGTTGAGGTGTTTACCCCATATAAGAAAC566300.37866326451464505No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAC555660.37154870132475304No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAA554070.3704855288179929No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAT548440.36672096201732635No Hit
GCATAGCTCTTAAACCGTCATCGTTGAGGTGTTTACCCCATATAAGAAAT537310.3592787544700052No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAC490880.32823277994870026No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAT477190.31907879779929976No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAG475750.31811592458563015No Hit
GCATAGCTCTTAAACCGTCATCGTTGAGGTGTTTACCCCATATAAGAAAG474590.317340276719063No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAA417330.2790526932366181No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAT412800.2760236545852825No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAC412210.2756291440324595No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAG409280.2736699645074235No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAACC401500.26846777450579196No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAG375040.2507749792046132No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAT354730.23719445492014837No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAA352830.23592399720766769No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAGC350570.23441282119176957No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAACG335170.22411542710113644No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAC331700.22179517012097433No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAC320560.21434627595411376No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAA316150.2113974767372506No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAG297210.19873301932967977No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAAG293190.19604499827485214No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAT291060.19462074831296589No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAC290890.19450707578079657No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAC290390.19417274480382796No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAA288750.19307613919937092No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAACA288510.19291566033042598No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAGG287820.1924542835822093No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAA287350.19214001246385884No Hit
GCATAGCTCTTAAACCCAGCATGTTGTACCAAAGGCCCCATATAAGAAAC285220.19071576250197256No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAA283130.18931825901824376No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAC281710.18836875904365294No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAC279920.18717185414610532No Hit
GCATAGCTCTTAAACCCTCGATATCTGGCAGGGAGCCCCATATAAGAAAC272030.1818961113295407No Hit
GCATAGCTCTTAAACCCTGGCAGAAACTTCAGCTGCCCCATATAAGAAAC267090.17859292127709084No Hit
GCATAGCTCTTAAACCCAGCATGTTGTACCAAAGGCCCCATATAAGAAAA263970.17650669598080673No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAA262420.17547026995220405No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAATG262340.17541677699588906No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAC262230.17534322418095596No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAGA262090.17524961150740476No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAT261080.1745742629339282No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAG259600.1735846432421011No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAT258770.1730296538203332No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAT256530.17153185104351384No Hit
GCATAGCTCTTAAACTTGATTTGTCAAGGCAGTGCCCCCATATAAGAAAC255190.170635844025238No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAA254150.16994043559314329No Hit
GCATAGCTCTTAAACCCAGCATGTTGTACCAAAGGCCCCATATAAGAAAT253830.16972646376788336No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAA253020.16918484758519423No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAG252060.1685429321094145No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAAC251800.16836908000139084No Hit
GCATAGCTCTTAAACCCTCGATATCTGGCAGGGAGCCCCATATAAGAAAA250500.16749981946127243No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAT245060.163862298431854No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAG244780.16367507308475157No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAATA240720.16096030555176646No Hit
GCATAGCTCTTAAACCCTCGATATCTGGCAGGGAGCCCCATATAAGAAAG240410.16075302034604594No Hit
GCATAGCTCTTAAACGTATGACCCTCTACAGGACACCCCATATAAGAAAA238580.15952936897034084No Hit
GCATAGCTCTTAAACCCTCGATATCTGGCAGGGAGCCCCATATAAGAAAT233350.1560322669512492No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAT231070.15450771769627236No Hit
GCATAGCTCTTAAACGTATGACCCTCTACAGGACACCCCATATAAGAAAC230540.1541533268606856No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAG227600.15218746071611022No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAT227180.15190662269545657No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAC226900.15171939734835416No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAT226270.1512981403173737No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAGT225350.15068297131975147No Hit
GCATAGCTCTTAAACCCTTCTCTCGCACTTCTATCCCCCATATAAGAAAC224150.1498805769750268No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAACT222190.14856999954530986No Hit
GCATAGCTCTTAAACCCTGGCAGAAACTTCAGCTGCCCCATATAAGAAAT218880.1463567284777777No Hit
GCATAGCTCTTAAACGTATGACCCTCTACAGGACACCCCATATAAGAAAT217340.14532698906871436No Hit
GCATAGCTCTTAAACCCTTCTCTCGCACTTCTATCCCCCATATAAGAAAA216920.14504615104806076No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAG216640.14485892570095832No Hit
GCATAGCTCTTAAACCCAGCATGTTGTACCAAAGGCCCCATATAAGAAAG214000.14309365814256408No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAG213550.14279276026329232No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAA212680.14221102436336697No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAG209370.13999775329583478No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAC209010.13975703499241737No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGACCCCCCCATATAAGAAAC208810.13962330260162994No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGACCCCCCCATATAAGAAAA206250.13791152799955067No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAATC201750.13490254920683317No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAC200640.13416033443796288No Hit
GCATAGCTCTTAAACCCTTCTCTCGCACTTCTATCCCCCATATAAGAAAT200200.13386612317823052No Hit
GCATAGCTCTTAAACTTGATTTGTCAAGGCAGTGCCCCCATATAAGAAAT192290.12857700712258716No Hit
GCATAGCTCTTAAACGTATGACCCTCTACAGGACACCCCATATAAGAAAG191130.12780135925601996No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGACCCCCCCATATAAGAAAT190480.12736672898596077No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAC190310.12725305645379145No Hit
GCATAGCTCTTAAACCCTGGCAGAAACTTCAGCTGCCCCATATAAGAAAA186570.12475226074606625No Hit
GCATAGCTCTTAAACCAGGTATGGGGGAGGCTTCTCCCCATATAAGAAAC185520.12405016569443218No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAC185220.12384956710825101No Hit
GCATAGCTCTTAAACTTGATTTGTCAAGGCAGTGCCCCCATATAAGAAAA184770.12354866922897928No Hit
GCATAGCTCTTAAACCCTGGCAGAAACTTCAGCTGCCCCATATAAGAAAG183240.12252561643945534No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAA183110.1224386903854435No Hit
GCATAGCTCTTAAACCAGGTATGGGGGAGGCTTCTCCCCATATAAGAAAA182850.12226483827741982No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAAA182070.1217432819533488No Hit
GCATAGCTCTTAAACCCTTCTCTCGCACTTCTATCCCCCATATAAGAAAG180650.12079378197875795No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAA180180.12047951086040745No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAT180160.12046613762132871No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAA177120.11843340528135957No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAT174720.11682861659191027No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAA173630.11609977506211869No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAG173390.11593929619317375No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAT169760.11351205330038168No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAG167850.1122349089683616No Hit
GCATAGCTCTTAAACCAGGTATGGGGGAGGCTTCTCCCCATATAAGAAAT166240.11115836322252268No Hit
GCATAGCTCTTAAACTTGATTTGTCAAGGCAGTGCCCCCATATAAGAAAG165860.11090427168002653No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAT165300.1105298209858217No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGACCCCCCCATATAAGAAAG164710.11013531043299873No Hit
GCATAGCTCTTAAACCATTCCTTGGATTACAGCTGCCCCATATAAGAAAC163170.10910557102393542No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAG158430.10593611336227303No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCCATATAAGAAA154680.10342863103500848No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCATATAAGAAAAT153750.10280677541784686No Hit
GCATAGCTCTTAAACCATTCCTTGGATTACAGCTGCCCCATATAAGAAAA152470.10195088811680722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTC207.8550517E-444.0050844
CTAGTAA650.044.0036137
CACCACG302.5283389E-644.0036113
CAACCGT351.4467878E-744.00331512
CGCTCGA1150.044.00301715
GATCGTA600.044.002436
TCGTCTG454.802132E-1044.0024342
ATCGTGG551.8189894E-1244.00213620
TTCTTCT207.8577694E-444.00198741
GTTCTAA254.4443157E-544.00184240
TCTTCTT207.857899E-444.00184240
TCGTTTT351.4477882E-743.99948521
CTGATGC1950.043.9978722
CCGCGTC408.3273335E-943.99786822
GTCATTC408.3273335E-943.9977223
CTCGTCG408.3273335E-943.9977223
CGCGCTC254.4469492E-543.9974253
TTCGTTC2600.043.99683824
TACATGG650.043.99683824
TCAATGG650.043.99683824
Produced by FastQC (version 0.11.5)