FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_GO3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_GO3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11253128
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTGGAAAGGACGAAACACCGGGTTCCCAAGGACCTATATGGTTTAAG7236836.430949687944542No Hit
CTTGTGGAAAGGACGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA5855615.2035398513195625No Hit
CTTGTGGAAAGGACGAAACACCGTTTTAGGAGATTAGATCCTGGTTTAAG4825394.288043288941528No Hit
CTTGTGGAAAGGACGAAACACCGGACCTGTCACCACAACAAAGGTTTAAG2874212.554143168015151No Hit
CTTGTGGAAAGGACGAAACACCGCAGCTGTAATCCAAGGAATGGTTTAAG1967201.7481361626740584No Hit
CTTGTGGAAAGGACGAAACACCGCAACCAGCCAGAAATTGCTGGTTTAAG1638651.456172896993618No Hit
CTTGTGGAAAGGACGAAACACCGACAGTTCAGAATTCATCTGGGTTTAAG1628091.4467888395120005No Hit
CTTGTGGAAAGGACGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTG1480031.3152165335718211No Hit
CTTGTGGAAAGGACGAAACACCGATCGTTTCCGCTTAACGGCGGTTTAAG1442951.282265695369323No Hit
CTTGTGGAAAGGACGAAACACCGTACTAACGCCGCTCCTACAGGTTTAAG1442621.2819724435730226No Hit
CTTGTGGAAAGGACGAAACACCGGACTCCGGGAAAGATGGTCCGTTTAAG1395421.2400285502839745No Hit
CTTGTGGAAAGGACGAAACACCGTCGCCGTCAGACACCTCCAGGTTTAAG1364261.2123384715787469No Hit
CTTGTGGAAAGGACGAAACACCGCAGTGAGCAACTGGTAATGGGTTTAAG1307131.161570365146473No Hit
CTTGTGGAAAGGACGAAACACCGAAGGCTTTCAGGTGGTAGGGGTTTAAG1233661.0962818515882873No Hit
CTTGTGGAAAGGACGAAACACCGCGGGCCTCCAGAAAACATTGGTTTAAG1227031.0903901564080671No Hit
CTTGTGGAAAGGACTTATCAAACTGGTTTCAAAGCACAGAGCTCAAGTAA1062720.9443774211046032No Hit
CTTGTGGAAAGGACGAAACACCGACAGTACTGAGAATTCAGTGGTTTAAG1040930.9250139161306972No Hit
CTTGTGGAAAGGACGAAACACCGCACCCTGCTGAGAAATGAAGGTTTAAG952620.8465379581570565No Hit
CTTGTGGAAAGGACGAAACACCGATGATGCCAATGTCCTGGTGGTTTAAG950880.8449917214129262No Hit
CTTGTGGAAAGGACGAAACACCGGACAGAGAGCAAGTTTTCTGGTTTAAG944920.839695416243377No Hit
CTTGTGGAAAGGACGAAACACCGCCACATTTATAAGAGTTGCGGTTTAAG931100.8274143864710327No Hit
CTTGTGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAA916380.8143335790724144No Hit
CTTGTGGAAAGGACGAAACACCGTGTGAACTTCTCCTCTGTCAGTTTAAG904380.8036698773887581No Hit
CTTGTGGAAAGGACGAAACACCGAGGAGCCAGATCCCAAGACGGTTTAAG890300.7911578007466012No Hit
CTTGTGGAAAGGACGAAACACCGAGTGTACGACGCGCACACCAGTTTAAG816600.7256648995728121No Hit
CTTGTGGAAAGGACGAAACACCGACACGTAATCCCACAGCAGGGTTTAAG746180.663086743525889No Hit
CTTGTGGAAAGGACGAAACACCGACCTAAACTGGATATCAGAAGTTTAAG742930.660198657653232No Hit
CTTGTGGAAAGGACGAAACACCGTGAAGCCTGTGGATGTCGGGGTTTAAG742030.6593988800269578No Hit
CTTGTGGAAAGGACGAAACACCGGAAGCAGCTGCGCTACACGGGTTTAAG726350.6454649764936469No Hit
CTTGTGGAAAGGACGAAACACCGTGTGCAACCGTACACGAAGGGTTTAAG724720.6440164903482837No Hit
CTTGTGGAAAGGACGAAACACCGCCGGCCGGAGACGCAAGAAGGTTTAAG683550.6074311071552727No Hit
CTTGTGGAAAGGACGAAACACCGGCACTGCCTTGACAAATCAAGTTTAAG672100.5972561584654507No Hit
CTTGTGGAAAGGACGAAACACCGCAAGTGCAAGGTGTACTGGGGTTTAAG648210.576026505696905No Hit
CTTGTGGAAAGGACGAAACACCGCCATATCGGGGCGAGACATGGTTTAAG627070.5572406178975303No Hit
CTTGTGGAAAGGACGAAACACCGTCACAATGTACTTTATGTGGGTTTAAG626600.5568229562482538No Hit
CTTGTGGAAAGGACGAAACACCGCTTCAAGATGCCGACAAAAAGTTTAAG618190.5493494786516246No Hit
CTTGTGGAAAGGACGAAACACCGGTTGCCGTAGAGCTTGCCCAGTTTAAG615670.5471101012980569No Hit
CTTGTGGAAAGGACGAAACACCGATGGCAGAGATCTGATGCAGGTTTAAG554740.4929651559992919No Hit
CTTGTGGAAAGGACGAAACACCGCCAGGCTCAACATTAGCAGGGTTTAAG552060.4905835959566087No Hit
CTTGTGGAAAGGACGAAACACCGTGGCCCTGAGCAATAGCACGGTTTAAG522310.4641465021992108No Hit
CTTGTGGAAAGGACGAAACACCGAGGCTAAGTGTAGACACGTGGTTTAAG505370.4490929099891159No Hit
CTTGTGGAAAGGACGAAACACCGCTTTCTCCCGAGCGGCGCGGGTTTAAG505040.44879965819281536No Hit
CTTGTGGAAAGGACGAAACACCGGATTTTGCAGGTCCACTGCGGTTTAAG501930.4460359821731344No Hit
CTTGTGGAAAGGACGAAACACCGGGCTTGAACGGATACTCCGGGTTTAAG500640.4448896342421414No Hit
CTTGTGGAAAGGACGAAACACCGGGGTGCAGAGTCCGTGACAGGTTTAAG468340.41618650387696643No Hit
CTTGTGGAAAGGACGAAACACCGTAAACACCTCAACGATGACGGTTTAAG450630.4004486574755037No Hit
CTTGTGGAAAGGACGAAACACCGGTTGCCGAGAGCTTGCCCAGTTTAAGA449450.3994000601432775No Hit
CTTGTGGAAAGGACGAAACACCGGCTGGATTTTTTTCGGGTAGGTTTAAG423120.37600212136572164No Hit
CTTGTGGAAAGGACGAAACACCGCCCAGGTAGATGGTATAGCGGTTTAAG415100.3688752140738113No Hit
CTTGTGGAAAGGACGAAACACCGCAGCTGAAGTTTCTGCCAGGGTTTAAG410840.36508959997611334No Hit
CTTGTGGAAAGGACGAAAATGGAAGAGACGCCCCTGCTAACGGGAATGCT406680.3613928500591125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG378840.3366530621530298No Hit
CTTGTGGAAAGGACGAAACACCGCTCCCTGCCAGATATCGAGGGTTTAAG368460.3274289601966671No Hit
CTTGTGGAAAGGACGAAACACCGGACCTAAAATCATTAACATCGTTTAAG361590.32132399098277387No Hit
CTTGTGGAAAGGACGAAACACCGTTCCGGGCACGGCAGCGCCGGTTTAAG355450.3158677302879697No Hit
CTTGTGGAAAGGACGAAACACCGGAAGCCTGCTGAGGGACTGGGTTTAAG339500.30169389346677655No Hit
CTTGTGGAAAGGACGAAAACTGGAAATAAAATCAGGTACAACTCCAAAAG331030.2941670973617291No Hit
CTTGTGGAAAGGACGAAACACCGCCTTTGGTACAACATGCTGGGTTTAAG328150.2916078089576516No Hit
CTTGTGGAAAGGACGAAACACCGTGTCCGAATCAAAACTTGTGGTTTAAG320370.28469417569941446No Hit
CTTGTGGAAAGGACGAAACACCGAGAAGCCTCCCCCATACCTGGTTTAAG310000.2754789601611214No Hit
CTTGTGGAAAGGACGAAACACCGTGGGTACTCGTCCTCCTTGCGTTTAAG303320.26954283289055275No Hit
CTTGTGGAAAGGACGAAACACCGACGGAGGCTAAGCGTCGCAAGTTTAAG300710.26722347777435745No Hit
CTTGTGGAAAGGACGAAACACCGGCACGTCCTCGATACCCCCAGTTTAAG292400.2598388643584255No Hit
CTTGTGGAAAGGACGAAACACCGGATAGAAGTGCGAGAGAAGGGTTTAAG292300.25975000017772837No Hit
CTTGTGGAAAGGACGAAACACCGACGGTCAATGAGCACATACAGTTTAAG286200.2543292851552031No Hit
CTTGTGGAAAGGACGAAACACCGGCCGACAAAAAAGGAAAGTCGTTTAAG282360.25091690061643307No Hit
CTTGTGGAAAGGACGACAAGGTGGGGACAGACTTGCTGGAGGAGGAGATC275490.24481193140253982No Hit
CTTGTGGAAAGGACGAAACACCGACGCTCCTCTTTCTCCCGAGGTTTAAG268820.23888469055004086No Hit
CTTGTGGAAAGGACGAAACACCGACCTGAAACCATTCACCACGGTTTAAG265720.23612990094842964No Hit
CTTGTGGAAAGGACCAGTTCGAATGCCTACCAAGACTTTGAGAATCACTA253790.2255284041912613No Hit
CTTGTGGAAAGGACGAAACACCGGAATAAAAAAAGCAAAGCCAGTTTAAG250130.22227597517774614No Hit
CTTGTGGAAAGGACGAAACACCGCAGTTGGAATGTAAAGGTGAGTTTAAG244200.21700632926240598No Hit
CTTGTGGGGATCTGGCACTGTGGTTCCTGCATGAAGACAGTGGCTGGCGG236420.2100926960041688No Hit
CTTGTGGAAAGGACGAAACACCGGGATACGTGGACCCTTCTGGGTTTAAG226770.20151730256689515No Hit
CTTGTGGAGAAGAAAATTGACAAATACACAGAGGTCCTCAAGACCCACGG224560.1995534041734885No Hit
CTTGTGGAAAGGACGAAACACCGTGGGGGCAAATACGTAGACTGTTTAAG223030.1981937822088223No Hit
CTTGTGGAAAGGACGAAACACCGCCCTCCGGACGTCACCACGGGTTTAAG219060.194665874235146No Hit
CTTGTGGAAAGGACGAAACACCGAGATGTTTGGAGCTTTGGAGGTTTAAG211200.1876811496323511No Hit
CTTGTGGAAAGGACGAAACACCGCGCTTCCGCGGCCCGTTCAAGTTTAAG210860.18737901141798086No Hit
CTTGTGGAAAGGACGAAACACCGGTAAGACCAAAGAAAAGGAGGTTTAAG200090.1778083391568993No Hit
CTTGTGGAAAGGACGAAACACCGAACCACGGCATTGAGAGGTGGTTTAAG198250.176173238232072No Hit
CTTGTGGAAAGGACGAAACACCGGCTGTGGTCATCAGGCAGAGGTTTAAG193350.17181889337791234No Hit
CTTGTGGAGAATTTTGATGAGGCTTCCAAGAATGAGGCAAACTGAATTGA191010.16973947154959937No Hit
CTTGTGGAAAGGACGAAACACCGAGCAGGATAGTCCTTCCGAGGTTTAAG190780.16953508393399594No Hit
CTTGTGGAAAGGACGAAACACCGTGCGGCGTTGCTTGAAGGTGGTTTAAG188100.16715352389131272No Hit
CTTGTGGAAAGGACGAAACACCGCAGGTGCCTTAGTGACCACGGTTTAAG186970.16614935864943506No Hit
CTTGTGGAAAGGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAAT186780.1659805167061105No Hit
CTTGTGGAAAGGACGAAACACCGGCATCAATGAATCTAAAGTGGTTTAAG179140.15919129330084933No Hit
CTTGTGGAAAGGACGAAACACCGTAAACACTCAACGATGACGGTTTAAGA175480.15593886428733414No Hit
CTTGTGGAAAGGACGAAACACCGGAATAGAGTTCAAGGACAAGGTTTAAG174980.15549454338384847No Hit
CTTGTGGAAAGGACGAAACAAAGTACAGTCCTCGCCCCAGTCATTGACCT172130.15296191423398012No Hit
CTTGTGGAAAGGACGAAAGGAACGCAAGAACAGAATGAAGAAAGTCAGGG167410.14876752490507528No Hit
CTTGTGGAAAGGACGAAACACCGGGACGTAGATGGAATAGACAGTTTAAG166770.14819879414861362No Hit
CTTGTGGAAAGGACGAAACACCGAGCTGAAGTCCTGTCATGTGGTTTAAG162100.14404883691005738No Hit
CTTGTGGAAAGGACGAAACACCGGTATAAAATACTCACGCAGAGTTTAAG145710.1294839976937968No Hit
CTTGTGGAAAGGACGAAACACCGAGCTCTGCCGTCCTGCAAGGGTTTAAG144330.1282576720001763No Hit
CTTGTGGAAAGGACGAAATACCACCGGAACTGGTCGAATGAGGCACCTAA141820.1260271810646782No Hit
CTTGTGGAAAGGACGAAACACCGGGTGTACAGCCCCACTACGGGTTTAAG140730.1250585614950794No Hit
CTTGTGGAAAGGACGGGAGCGTGACAGGAGGCGGTCGAGAGATCGTGAAA138140.1227569792150236No Hit
CTTGTGGAAAGGACGAAACACCGACAGCTGAAACAGCTTCTTCGTTTAAG137380.12208161144172537No Hit
CTTGTGGAAAGGACGAAACACCGTGTCCTGTAGAGGGTCATACGTTTAAG136340.12115742396247514No Hit
CTTGTGGAAAGGACATTGGAAAGCCCATCGAGAAGGGGCCTAGGGCGAAA136280.12110410545405687No Hit
CTTGTGGAAAGGACGAAACACCGGGCACGAGACATCTTCTCGTGTTTAAG131930.11723851359373146No Hit
CTTGTGGAAAGGACGAAACACCGTACATGGTATAGTGTTTATTGTTTAAG131470.11682973836252461No Hit
CTTGTGGAAAGGACGAAACACCGGGGCAGAAGTTGCTGTCCTGGTTTAAG127750.11352399084059116No Hit
CTTGTGGAAAGGACGAAACACCGTAAACTGGATATCAGAAAGGGTTTAAG121180.10768561416878933No Hit
CTTGTGGAGAAGATTGGGGGCTGGCTGAGGAAAAATGTGCTGGTGGTAGC121070.10758786357002248No Hit
CTTGTGGAAAGGACGAAACACCGAAAGTATTACTCCAGGCCAAGTTTAAG120060.1066903353449814No Hit
CTTGTGGAAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAG116860.10384668156267307No Hit
CTTGTGGAAAGGACGAAAGAGCCGTGGCCTTGGAAAGGGCCACAAGTTCC116600.1036156346928605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTC207.857303E-444.00242620
TCGCGGG700.044.00144638
TCGGCTA254.4454144E-543.9998836
CGCGATC207.859884E-443.9994922
TTGTGGA10580800.043.8745652
TGTGGAA10485500.043.871613
GTGGAAA10440050.043.834134
CTTGTGG10662000.043.811061
AAGGACG9164300.043.7442639
GGACGAA8880650.043.73921211
ACGAAAC8274000.043.7291913
AAAGGAC9653800.043.7255178
GGAAAGG10210200.043.7249646
TTCGTAC8050.043.72367532
AGGACGA9031650.043.72330510
CGAAACA8123850.043.72096314
GACGAAA8571650.043.71758312
TGGAAAG10324550.043.7035185
CGCCATA74200.043.67334722
CGCGCTT25750.043.65774522
Produced by FastQC (version 0.11.5)