FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_ADT5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_ADT5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17140458
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG842650.4916146348014738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCT1952350.043.8250921
TAACCAG5025550.043.793662
TTAACCA4990050.043.777021
GTACCCC4927200.043.69999311
GTCCGAT6345600.043.6905947
TCCGATT6345800.043.6850938
CCAGTTG5041250.043.68395
TACCCCC4894750.043.68302512
ACCAGTT5008650.043.682144
ATTCCCC6244250.043.6803112
GCAGTTG6460400.043.6707341
GATTCCC6318800.043.66927311
CATTCTG1955850.043.6649672
GTTGTCC6381700.043.6558234
AGTTGTC6456450.043.6475683
TTCCCCA6260750.043.6406513
CCGATTC6300700.043.626259
TTGTCCG6383550.043.621445
TTGTACC4962000.043.6200269
TGTCCGA6390100.043.611096
Produced by FastQC (version 0.11.5)