FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n02_ADT3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n02_ADT3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394191
Sequences flagged as poor quality0
Sequence length50
%GC17

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4696946.352202695332052No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4669326.314849048394882No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3578624.839772194145377No Hit
CTCTATTCAGACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1818082.4587950189547443No Hit
CTCTATTCAGACCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770392.3942984431968286No Hit
CTCTATTCAGACCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1642692.2215953036647282No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1412921.9108513696765477No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1333431.8033480606600507No Hit
GTTGTCCGACAATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1266301.7125605762685872No Hit
GTCTTTGTCAGTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA949491.2841026151474855No Hit
TGATAGAAACAGACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870261.1769509335098323No Hit
TGATAGAAACAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA817441.1055164790847303No Hit
TGATAGAAACAGACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA741541.002868332722268No Hit
GTCTTTGTCAGTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA648490.8770263034860745No Hit
GTCTTTGTCAGTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA592970.8019403339729796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG569580.7703073940069982No Hit
AAGCGCTTGGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524330.7091107059582312No Hit
TCAACGCTTGGCTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA495510.6701341634263979No Hit
TCAACGCTTGGCTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA457540.6187830419852557No Hit
AAGCGCTTGGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA454770.6150368579875742No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT427240.5778049282200041No Hit
TCAACGCTTGGCTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA369180.49928382969820495No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT364760.49330616425786133No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT301040.4071304081812331No Hit
GCATTCTGTCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300750.40673820841252273No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG289720.3918210930715747No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC269800.3648810262001617No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT255650.34574438231308874No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC221010.2988967961471377No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG203010.2745533622271862No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG202970.27449926570736405No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT202730.27417468658843136No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC187850.25405078121460484No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT171620.23210111829678187No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG164650.22267479971777845No Hit
CTCTATTCAGACCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT157420.21289685375993128No Hit
CTCTATTCAGACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT152780.206621657460566No Hit
CTCTATTCAGACCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT141790.19175863863944007No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT130170.17604359963111582No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT129380.17497519336462905No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC128440.1737039251488094No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA121430.16422351004998384No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA116130.15705572117355365No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT115010.15554101861853445No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG111180.150361276845567No Hit
CTCTATTCAGACCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110550.1495092566583687No Hit
GTTGTCCGACAATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT107110.14485695595366688No Hit
CTCTATTCAGACCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG106190.14361273599775823No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG103060.13937968332167777No Hit
CTCTATTCAGACCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC100790.1363097058217728No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA100720.1362150369120841No Hit
CTCTATTCAGACCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT98620.1333749696214231No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC98140.1327258113835577No Hit
CTCTATTCAGACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC95180.12872266891672124No Hit
CTCTATTCAGACCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC92750.1254363053375278No Hit
GCATTCTGTCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92270.12478714709966243No Hit
CTCTATTCAGACCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG91680.12398922343228623No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA91390.1235970236635759No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG90900.122934341295755No Hit
CTCTATTCAGACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG90380.12223108653806752No Hit
CTCTATTCAGACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT89330.12081105289273701No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT89120.12052704616367091No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC86900.11752468931354357No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC86690.11724068258447747No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA84060.11368383640617344No Hit
CTCTATTCAGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83690.11318344359781889No Hit
CTCTATTCAGACCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT83610.11307525055817465No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG83260.11260190600973115No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG82860.11206094081151001No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT81720.11051918999657975No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA81120.10970774219924803No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC79290.1072328264173863No Hit
GTCTTTGTCAGTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT79010.1068541507786315No Hit
GAATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78890.10669186121916516No Hit
GAATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78360.10597508233152213No Hit
TGATAGAAACAGACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT78230.10579926864210028No Hit
GCATTCTGTCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77480.10478495889543563No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT76350.10325673221046089No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA75030.10147154705633112No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA74050.1001461823206893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC1250.044.00423410
GTGCATC207.855541E-444.0042311
AGCGTAC850.044.0042310
GTACCTT2100.044.0042310
CCCCTAC408.3164196E-944.0042310
AGTCCAT408.3164196E-944.0042310
AGTCCAC351.446424E-744.0042310
TCGCAGA1650.044.0042311
GCCCAGC1050.044.0042310
ACTGCAG207.855541E-444.0042310
GGACTTA850.044.0042311
GGACTGA800.044.0042311
GTCTCTA900.044.0042311
GCTATCA207.855541E-444.0042311
CAACCTA1050.044.0042310
GTCTCCA600.044.0042311
CATCTAT302.5279187E-644.0042310
CATCTAG207.855541E-444.0042310
GTCTAGC600.044.0042310
TCTAGCA600.044.0042311
Produced by FastQC (version 0.11.5)