FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28632967
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGG2190620.7650691596159072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGC1683280.5878817937379665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGT776680.27125376144218655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC183250.019.68135621
GGGAGAT36650.017.5949691
GACTAAC30100.017.2018241
GCGCGAT24700.017.0931931
TGGCCAG33050.016.7468551
GGGGGGT104600.016.63402721
GGGAATG24500.016.5897181
GGCTCGA26850.016.5850071
GACCTGG26350.016.4612791
CCTTACG28100.016.3704991
CCACTAC35800.016.2525251
GAACCTA36650.016.2194671
CAACTAG41350.016.2046341
GGGACCT19150.016.1788391
GACTGCG34650.016.1554131
CTGATCC31050.016.0666791
CTCGGGA26950.016.0168511
CCCTCCT46350.015.9520691
GCATGCG28350.015.929771
CGATTGA26250.015.9238421
Produced by FastQC (version 0.11.5)