FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_cDNA5_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_cDNA5_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44520511
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCTA147600.017.6979941
CAATGGA162850.017.601718
GACTAAC113400.017.4595451
TGACGGC105050.017.4375321
GACCTGG142900.017.1554971
GGATTAC133600.017.0367621
TGGCTGG171300.016.9846651
CCTTACG126050.016.9822791
ACTGATG126600.016.9665781
AGAAACC133200.016.9557158
TGACTAG122000.016.9519821
GGGCATC126000.016.9056551
CCCTCCT241400.016.7908291
CTTTGCG131150.016.778391
GGGAATG120450.016.7777181
CTACCCA139800.016.7770711
TTGACTT189050.016.6233881
CGATTGA119300.016.5960791
GAATGAA140150.016.5852851
GGTATTG136250.016.5666431
Produced by FastQC (version 0.11.5)