FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_cDNA5_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_cDNA5_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50061711
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCTA168550.017.7422521
GACTAAC125300.017.6126631
CAATGGA176850.017.4016278
TGACGGC120950.017.1692311
ACTGATG147050.017.136231
TGGCTGG193500.017.0396391
GGGCATC140000.017.0087931
TGACTAG135250.016.9926091
GACCTGG151500.016.8894181
GGATTAC138300.016.8760931
CTTTGCG152500.016.861321
CTACCCA154500.016.8334141
GAATGAA157050.016.8276231
GGGAATG133400.016.8266931
CGCTATC130850.016.7933751
TTGACTT219750.016.78711
AGAAACC150500.016.7647238
CCTTACG143300.016.7563761
GCGCGAT105850.016.7506481
CCCTCCT271550.016.7334981
Produced by FastQC (version 0.11.5)