FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_cDNA5_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_cDNA5_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36259687
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA134400.017.6949148
GATGCTA122300.017.4432371
GACTAAC93100.017.3181841
ACTGATG101350.017.2661251
TGACGGC85350.017.081641
CGATTGA94750.017.01661
CTACCCA106850.016.977021
TGACTAG98150.016.9408131
GACCTGG112050.016.9203361
AGAAACC108600.016.8808358
TGGCTGG137400.016.8487821
GGGAATG98700.016.8038431
CCCTCCT192800.016.7961651
CCTTACG104450.016.7838481
GGGCATC100200.016.7724271
CTCGAAA86000.016.7571181
GGATTAC104000.016.7453751
CTTTGCG107550.016.7004951
TATGCCC101550.016.6839181
GAATGAA112750.016.6569161
Produced by FastQC (version 0.11.5)