FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_cDNA5_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_cDNA5_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62853322
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCTA209050.017.8262041
ACTGATG178150.017.3276311
CAATGGA228450.017.2445958
GACTAAC160800.017.1266371
TGACTAG163950.017.1243061
TGACGGC153900.017.0823521
GACCTGG192650.017.004811
CCCTCCT341750.016.9189831
CCTTACG177500.016.911781
CGATTGA159650.016.8552671
GGGCATC180350.016.8367161
GGGAATG168000.016.8302751
CTGCGGA163050.016.787231
GGATTAC185750.016.7656861
AGAAACC191450.016.7602048
ACTGCTC238300.016.7268121
GAATGAA200700.016.6995541
TGGCTGG236150.016.6967431
TTGACTT268350.016.6346151
CTACCCA195400.016.6096061
Produced by FastQC (version 0.11.5)