FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_cDNA3_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_cDNA3_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44015339
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC96100.018.4059961
CGCTGGA137100.018.2722321
CTGCGGA96750.018.1737731
ATAGACC146950.018.0695841
GACTAAC119500.018.036411
CAATGGA106500.017.8249428
GACTGCG145700.017.6839241
GGGAGAT140400.017.6557241
CACCGAA88200.017.5472158
GATGCTA127400.017.5280841
GCGCGAT91150.017.446561
CGTGAGC111250.017.3817861
AGAAACC130100.017.3274258
ACTGAAC100000.017.278521
ACGAGCC102200.017.2765671
GGCTCGA106200.017.2687281
TGACTAG119650.017.2676121
GCGGAAG39600.017.261223
GATCTAG131500.017.253161
CTAATGG134000.017.2056221
Produced by FastQC (version 0.11.5)