FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_cDNA3_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_cDNA3_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45890449
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC101900.018.3275381
CGCTGGA139450.018.2959751
CTGCGGA101800.018.190771
GACTGCG147600.018.1610471
CAATGGA114400.017.9340368
GACTAAC123650.017.8135641
CGGACTG93100.017.7470251
ACTGAAC104500.017.7408711
GGGAGAT152700.017.6920621
GCGCGAT96850.017.6021391
ATAGACC159050.017.5470621
GATGCTA130950.017.5183681
ACGAGCC109850.017.4888081
CACCGAA85850.017.452728
CGTGAGC121050.017.3978711
GGCGACT96400.017.3029441
ACTGATG104050.017.3027551
GATCTAG140350.017.2431491
ATGCGAT113200.017.2403411
AGCTTGA115650.017.2020761
Produced by FastQC (version 0.11.5)