FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_cDNA3_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_cDNA3_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69476999
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA210550.018.4583321
CTGCGGA151050.018.392931
TGACGGC150700.018.1707881
GACTGCG224950.017.9257811
CAATGGA169500.017.8402738
ATAGACC229100.017.8394761
GACTAAC186950.017.737611
GGGAGAT216200.017.6795961
GCGCGAT148200.017.5630151
CGGACTG141500.017.5186861
CGTGAGC173650.017.4750611
GATGCTA203800.017.4204141
CACCGAA135250.017.381838
AGGGTGA159750.017.3057731
ACTGATG153900.017.2947391
AGCTTGA168000.017.2874851
ATGCGAT174000.017.2105161
TGGCTGG201550.017.2031781
ACTGAAC156650.017.1721691
TACGGGC176350.017.1479471
Produced by FastQC (version 0.11.5)