FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_cDNA3_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_cDNA3_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44391928
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA135050.018.2076341
TGACGGC102500.018.0767921
GACTGCG148350.018.0621531
CTGCGGA96700.018.0096261
CAATGGA108900.017.981648
GACTAAC116100.017.9406071
ATAGACC147600.017.7981151
GCGCGAT90300.017.773881
GATGCTA126700.017.616881
ATGCGAT107100.017.4965361
GGGAGAT140150.017.4626221
CACCGAA89700.017.2888628
GCGGAAG40150.017.2863223
ACTGAAC99750.017.2799431
CGTGAGC111900.017.2716731
ATGGGAG107150.017.2531011
GGCTCGA107450.017.2440321
ACTGATG97900.017.2202321
ACGAGCC103600.017.195421
AGGGTGA101600.017.1927431
Produced by FastQC (version 0.11.5)