FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_HTO5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_HTO5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8563618
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAC294600.020.4005249
ACACCCT164500.020.370641
GCATTCG292850.020.2893877
TCTATTG163800.020.239651
ACCCTAG101900.020.2376213
CAAGCAT299450.020.2146954
ATTGTCC123350.020.1744374
AAGCATT300600.020.1058045
GTCCTAT122750.020.1011457
TATTGTC128650.020.0941873
TTCGACA299700.020.0218410
ACAAGCA301900.019.9953
ATTGGTG95400.019.9851841
CCCTAGC101150.019.951694
TGTCCTA124600.019.8960846
CATTCGA302700.019.8372788
GAACTTG72800.019.6738972
CTATTCA127100.019.57840210
CCTATTC127100.019.5453599
CTATTGT128650.019.4827382
Produced by FastQC (version 0.11.5)