FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_HTO3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_HTO3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10267248
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAA353050.020.7651862
GCCGAAG358650.020.748063
ACGCCGA366800.020.7468171
CTGCGGA214200.020.73281
CCGAAGC351700.020.6953164
CGAAGCG352850.020.6695275
TGCGGAG201900.020.578892
GAAGCGT355700.020.5629526
GCGGAGT202900.020.4875183
AAGCGTC357150.020.4380117
AGCGTCA360750.020.4086888
CGGAGTG203200.020.3797594
GGCCCTA204600.020.32212310
GCGTCAA365050.020.182679
GTGGCCC206800.020.161788
GGAGTGG203150.020.157365
TGGCCCT206600.020.1508069
AGTGGCC203500.020.0966017
GAGTGGC205350.019.8953886
TGAAGAC52500.019.8798455
Produced by FastQC (version 0.11.5)